| Dissection and Staining |
| E_Staining_Uemura.pdf |
X-gal staining of embryos and imaginal discs, antibody staining of embryos (by UEMURA Tadashi) |
| E_Staining_ItoKei.pdf |
GFP, X-gal, fluorescent and DAB antibody staining of embryos, larvae, pupae and adult CNSs (by ITO Kei) |
| E_CNS_Dissection.pdf |
Preparation of dissection tools, How to dissect brains of pupae and adults (by ITO Kei) |
| E_Pupal_Injection.pdf |
Staging and BrdU microinjection of pupae (by ITO Kei) |
| E_DiI_Staining.pdf |
DiI microinjection and photoconversion (by ITO Kei) |
| Molecular Biology |
| E_Northern_blotting.pdf |
RNA electrophoresis and Northern blotting (by ITO Hiroki) |
| E_Southern_blotting.pdf |
RNA electrophoresis and Northern blotting (by ITO Hiroki) |
| E_Plasmid_preparation.pdf |
Miniprep (by ITO Hiroki) |
| E_Miniprep.pdf |
Miniprep (by ITO Kei) |
| E_Transformation.pdf |
E. coli transformation (by ITO Kei) |
| Text for Anatomy and Development |
| E_anatomical_terminology.pdf |
Name, definition and abbreviation of major brain regions (by ITO Kei) |
| Brain_Atlas.pdf |
Serial silver sections of the brain (frontal, horizontal and sagittal, total 9MB!) (by ITO Kei) |
Color Blind
Please feel free to use these items for your classes and seminars. Please don't forget, however, to mention Masataka Okabe and Kei Ito for reference. |
| color_blind.pdf |
How to make figures and presentations that are friendly to color blind people（1.4MB） |
| color_blind.ppt |
as above PowerPoint file（7.8MB |
| Miscreneous |
Zen and the Art of the Internet |
classic text of the Internet in its infancy: 1992 Brendan P. Kehoe |
| Electronic Handling of Japanese Text |
Classic textbook of Japanese text processing on computers: 1992 Ken R. Lunde |
| King James Bible |
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| Latin Bible |
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